| Item type |
*学術雑誌論文 / Journal Article(1) |
| 公開日 |
2020-10-24 |
| タイトル |
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タイトル |
Stromal Fibroblasts Produce Interleukin-11 in the Colon of TNBS-treated Mice(en) |
| 言語 |
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言語 |
eng |
| キーワード |
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主題 |
colitis, interleukin 11, mitogen-activated protein kinase/ERK kinase, stromal fibroblasts, trinitrobenzene sulfonic acid |
| 資源タイプ |
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資源タイプ |
journal article |
| ID登録 |
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ID登録 |
10.14994/tohojmed.2020-003 |
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ID登録タイプ |
JaLC |
| 著者 |
Takeda, Wakami
| en |
Takeda, Wakami
Department of Biochemistry, Faculty of Medicine, Toho University / Laboratory of Molecular Biology and Immunology, Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
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Nishina, Takashi
| en |
Nishina, Takashi
Department of Biochemistry, Faculty of Medicine, Toho University
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Deguchi, Yutaka
| en |
Deguchi, Yutaka
Department of Biochemistry, Faculty of Medicine, Toho University
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Kawauchi, Mika
| en |
Kawauchi, Mika
Department of Biochemistry, Faculty of Medicine, Toho University
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Mikami, Tetuo
| en |
Mikami, Tetuo
Department of Pathology, Faculty of Medicine, Toho University
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Yagita, Hideo
| en |
Yagita, Hideo
Department of Immunology, Juntendo University Graduate School of Medicine
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Nishiyama, Chiharu
| en |
Nishiyama, Chiharu
Laboratory of Molecular Biology and Immunology, Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
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Nakano, Hiroyasu
| en |
Nakano, Hiroyasu
Department of Biochemistry, Faculty of Medicine, Toho University / Host Defense Research Center, Faculty of Medicine, Toho University
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| 内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Original Article |
| 内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Introduction: Interleukin (IL) -11 is a member of the IL-6 cytokine family and is implicated to play a role in pleiotropic functions such as hematopoiesis, bone development, tissue repair, and tumor development. Although the protective function of IL-11 in trinitrobenzene sulfonic acid (TNBS)-induced colitis has been reported, whether Il11 expression is induced in the colon of TNBS-treated mice is still unclear. Methods: After inducing TNBS-induced colitis in C57BL/6 mice, Il6 and Il11 expressions in the colon were determined using quantitative PCR. Colonic sections were stained with hematoxylin and eosin (H&E) or immunostained with anti-IL-11 antibody along with antibodies against lineage-specific markers. To assess the contribution of the transforming growth factor (TGF)-β signal or mitogen-activated protein kinase/ERK kinase (MEK)/ERK pathway to upregulation of Il11 expression, we treated mice with TNBS in the presence of neutralizing antibody against TGF-β or a MEK inhibitor, trametinib. Subsequently, Il11 expression in the colon was determined using qPCR. Results: TNBS treatment increased Il11 expression in the colon. Immunohistochemical analysis revealed that IL-11+ cells appeared in the subepithelial tissues of the inflamed colon. IL-11+ cells expressed podoplanin, vimentin, and collagen IV but did not express α-smooth muscle actin, suggesting that these cells were fibroblasts, and not myofibroblasts. Moreover, TNBS administration induced ERK activation in the colon, and the blockade of the MEK/ERK pathway abolished Il11 expression in the colon. Conclusions: Stromal fibroblasts produced IL-11 in the colon of TNBS-treated mice in a MEK/ERK-dependent manner. |
| 書誌情報 |
ja : Toho Journal of Medicine
en : Toho Journal of Medicine
巻 6,
号 3,
p. 111-120,
発行日 2020-09-01
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| 出版者 |
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出版者 |
The Medical Society of Toho University(en) |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
21891990 |
| 著者版フラグ |
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出版タイプ |
VoR |
| レコードID |
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内容記述 |
TD45782806 |
| 資源タイプ(ローカル) |
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内容記述タイプ |
Other |
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内容記述 |
TOHO University Scholarly Publication |
| 出版地 |
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内容記述 |
Tokyo |
td45782806.pdf (2.5 MB)
