{"created":"2024-10-08T11:33:31.486381+00:00","id":2002941,"links":{},"metadata":{"_buckets":{"deposit":"ed4fc4bd-5075-4a51-a5e3-444fb3408d22"},"_deposit":{"created_by":82,"id":"2002941","owner":"82","owners":[82],"pid":{"revision_id":0,"type":"depid","value":"2002941"},"status":"published"},"_oai":{"id":"oai:toho.repo.nii.ac.jp:02002941","sets":["1726902243533:1727660392090:1727660862335"]},"author_link":[],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-09-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"120","bibliographicPageStart":"111","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"Toho Journal of Medicine","bibliographic_titleLang":"ja"},{"bibliographic_title":"Toho Journal of Medicine","bibliographic_titleLang":"en"}]}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Original Article","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"Introduction: Interleukin (IL) -11 is a member of the IL-6 cytokine family and is implicated to play a role in pleiotropic functions such as hematopoiesis, bone development, tissue repair, and tumor development. Although the protective function of IL-11 in trinitrobenzene sulfonic acid (TNBS)-induced colitis has been reported, whether Il11 expression is induced in the colon of TNBS-treated mice is still unclear. Methods: After inducing TNBS-induced colitis in C57BL/6 mice, Il6 and Il11 expressions in the colon were determined using quantitative PCR. Colonic sections were stained with hematoxylin and eosin (H&E) or immunostained with anti-IL-11 antibody along with antibodies against lineage-specific markers. To assess the contribution of the transforming growth factor (TGF)-β signal or mitogen-activated protein kinase/ERK kinase (MEK)/ERK pathway to upregulation of Il11 expression, we treated mice with TNBS in the presence of neutralizing antibody against TGF-β or a MEK inhibitor, trametinib. Subsequently, Il11 expression in the colon was determined using qPCR. Results: TNBS treatment increased Il11 expression in the colon. Immunohistochemical analysis revealed that IL-11+ cells appeared in the subepithelial tissues of the inflamed colon. IL-11+ cells expressed podoplanin, vimentin, and collagen IV but did not express α-smooth muscle actin, suggesting that these cells were fibroblasts, and not myofibroblasts. Moreover, TNBS administration induced ERK activation in the colon, and the blockade of the MEK/ERK pathway abolished Il11 expression in the colon. Conclusions: Stromal fibroblasts produced IL-11 in the colon of TNBS-treated mice in a MEK/ERK-dependent manner.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.14994/tohojmed.2020-003","subitem_identifier_reg_type":"JaLC"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The Medical Society of Toho University","subitem_publisher_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"21891990","subitem_source_identifier_type":"PISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1726887171532":{"attribute_name":"レコードID","attribute_value_mlt":[{"subitem_description":"TD45782806","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_1726887212651":{"attribute_name":"資源タイプ(ローカル)","attribute_value_mlt":[{"subitem_description":"TOHO University Scholarly Publication","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1726887247123":{"attribute_name":"出版地","attribute_value_mlt":[{"subitem_description":"Tokyo","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Department of Biochemistry, Faculty of Medicine, Toho University / Laboratory of Molecular Biology and Immunology, Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Takeda, Wakami","creatorNameLang":"en"}],"creatorType":"Author"},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Department of Biochemistry, Faculty of Medicine, Toho University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Nishina, Takashi","creatorNameLang":"en"}],"creatorType":"Author"},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Department of Biochemistry, Faculty of Medicine, Toho University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Deguchi, Yutaka","creatorNameLang":"en"}],"creatorType":"Author"},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Department of Biochemistry, Faculty of Medicine, Toho University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Kawauchi, Mika","creatorNameLang":"en"}],"creatorType":"Author"},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Department of Pathology, Faculty of Medicine, Toho University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Mikami, Tetuo","creatorNameLang":"en"}],"creatorType":"Author"},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Department of Immunology, Juntendo University Graduate School of Medicine","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Yagita, Hideo","creatorNameLang":"en"}],"creatorType":"Author"},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Laboratory of Molecular Biology and Immunology, Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Nishiyama, Chiharu","creatorNameLang":"en"}],"creatorType":"Author"},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Department of Biochemistry, Faculty of Medicine, Toho University / Host Defense Research Center, Faculty of Medicine, Toho University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Nakano, Hiroyasu","creatorNameLang":"en"}],"creatorType":"Author"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","displaytype":"detail","filename":"td45782806.pdf","filesize":[{"value":"2.5 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://toho.repo.nii.ac.jp/record/2002941/files/td45782806.pdf"},"version_id":"baa927fb-c4d1-43c5-a1cd-797eee1d0820"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"colitis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"interleukin 11","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mitogen-activated protein kinase/ERK kinase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"stromal fibroblasts","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"trinitrobenzene sulfonic acid","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Stromal Fibroblasts Produce Interleukin-11 in the Colon of TNBS-treated Mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Stromal Fibroblasts Produce Interleukin-11 in the Colon of TNBS-treated Mice","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"82","path":["1727660862335"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-10-24"},"publish_date":"2020-10-24","publish_status":"0","recid":"2002941","relation_version_is_last":true,"title":["Stromal Fibroblasts Produce Interleukin-11 in the Colon of TNBS-treated Mice"],"weko_creator_id":"82","weko_shared_id":-1},"updated":"2024-12-06T08:11:01.136682+00:00"}