| Item type |
*学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-03-12 |
| タイトル |
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タイトル |
LR11, an LDL Receptor Gene Superfamily Member, Represses the Norepinephrine-Induced Expression of Uncoupling Protein 1 in Primary Cultured Beige Adipocytes(en) |
| 言語 |
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言語 |
eng |
| キーワード |
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主題 |
LR11, adipogenesis, thermogenesis, UCP1, beige |
| 資源タイプ |
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資源タイプ |
journal article |
| ID登録 |
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ID登録 |
10.14994/tohojmed.2020-019 |
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ID登録タイプ |
JaLC |
| 著者 |
Nakamura, Shoko
| en |
Nakamura, Shoko
Department of Diabetes, Endocrinology and Metabolism, Toho University Graduate School of Medicine / Center for Diabetes, Endocrinology and Metabolism, Toho University Sakura Medical Center
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Jiang, Meizi
| en |
Jiang, Meizi
Department of Clinical-Laboratory and Experimental-Research Medicine, Toho University Sakura Medical Center / Department of Genome Research and Clinical Application, Chiba University Graduate School of Medicine
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Oka, Rena
| en |
Oka, Rena
Center for Diabetes, Endocrinology and Metabolism, Toho University Sakura Medical Center
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Yamaguchi, Takashi
| en |
Yamaguchi, Takashi
Center for Diabetes, Endocrinology and Metabolism, Toho University Sakura Medical Center
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Hiruta, Nobuyuki
| en |
Hiruta, Nobuyuki
Department of Clinical-Laboratory and Experimental-Research Medicine, Toho University Sakura Medical Center / Department of Surgical Pathology, Toho University Sakura Medical Center
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Ebinuma, Hiroyuki
| en |
Ebinuma, Hiroyuki
Tsukuba Research Institute, Sekisui Medical Co Ltd
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Schneider, J, Wolfgang
| en |
Schneider, J, Wolfgang
Department of Medical Biochemistry, Max Perutz Labs, Medical University of Vienna
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Tatsuno, Ichiro
| en |
Tatsuno, Ichiro
Center for Diabetes, Endocrinology and Metabolism, Toho University Sakura Medical Center
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Bujo, Hideaki
| en |
Bujo, Hideaki
Department of Clinical-Laboratory and Experimental-Research Medicine, Toho University Sakura Medical Center / Department of Genome Research and Clinical Application, Chiba University Graduate School of Medicine
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| 内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Original Article |
| 内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Introduction: The soluble form of LR11 (sLR11) inhibits thermogenesis via BMPs/TGFβ signaling pathways in adipocytes. Mice lacking LR11 are protected from diet-induced obesity associated with increased browning of subcutaneous white adipose tissue (WAT) and hypermetabolism. However, the mechanism underlying the recruitment of beige adipocytes has not been elucidated. In this paper, we have investigated the expression of LR11 in the differentiation and norepinephrine-induced activation of cultured beige adipocytes. Methods: LR11 mRNA levels were analyzed in cultured beige adipocytes differentiated from human immortalized adipose-derived stromal cells or from the subcutaneous WAT of wild-type (WT) and Lr11-/- mice. The concentrations of sLR11 were measured by an ELISA specific for murine sLR11. Results: In the course of browning adipogenesis of human stromal cells, LR11 mRNA levels decreased at the beginning and subsequently increased together with uncoupling protein (UCP) 1, β3-adrenergic receptor, lipoprotein lipase, and adiponectin. The LR11 transcript levels in the differentiated cells were transiently decreased by incubation with norepinephrine, and this change was in clear contrast to the increase of UCP1 mRNA levels. The mRNA levels of UCP1 in murine Lr11-/- beige adipocytes were significantly increased compared with those in WT beige adipocytes, regardless of norepinephrine stimulation. Finally, the amounts of sLR11 released from murine WT beige adipocytes were decreased by incubation with norepinephrine. Conclusions: LR11 potentially represses the norepinephrine-induced expression of UCP1 in Introduction: The soluble form of LR11 (sLR11) inhibits thermogenesis via BMPs/TGFβ signaling pathways in adipocytes. Mice lacking LR11 are protected from diet-induced obesity associated with increased browning of subcutaneous white adipose tissue (WAT) and hypermetabolism. However, the mechanism underlying the recruitment of beige adipocytes has not been elucidated. In this paper, we have investigated the expression of LR11 in the differentiation and norepinephrine-induced activation of cultured beige adipocytes. Methods: LR11 mRNA levels were analyzed in cultured beige adipocytes differentiated from human immortalized adipose-derived stromal cells or from the subcutaneous WAT of wild-type (WT) and Lr11<SUP>-/-</SUP> mice. The concentrations of sLR11 were measured by an ELISA specific for murine sLR11. Results: In the course of browning adipogenesis of human stromal cells, LR11 mRNA levels decreased at the beginning and subsequently increased together with uncoupling protein (UCP) 1, β3-adrenergic receptor, lipoprotein lipase, and adiponectin. The LR11 transcript levels in the differentiated cells were transiently decreased by incubation with norepinephrine, and this change was in clear contrast to the increase of UCP1 mRNA levels. The mRNA levels of UCP1 in murine Lr11<SUP>-/-</SUP> beige adipocytes were significantly increased compared with those in WT beige adipocytes, regardless of norepinephrine stimulation. Finally, the amounts of sLR11 released from murine WT beige adipocytes were decreased by incubation with norepinephrine. Conclusions: LR11 potentially represses the norepinephrine-induced expression of UCP1 in differentiated beige adipocytes. The sLR11-mediated repression of the key molecule in thermogenesis may be involved in the sympathetic activation of the recruitment of beige adipocytes in subcutaneous fat. |
| 書誌情報 |
ja : Toho Journal of Medicine
en : Toho Journal of Medicine
巻 7,
号 1,
p. 48-56,
発行日 2021-03-01
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| 出版者 |
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出版者 |
The Medical Society of Toho University(en) |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
21891990 |
| 著者版フラグ |
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出版タイプ |
VoR |
| レコードID |
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内容記述 |
TD42163805 |
| 資源タイプ(ローカル) |
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内容記述 |
TOHO University Scholarly Publication |
| 出版地 |
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内容記述 |
Tokyo |
td42163805.pdf (140.0 KB)
